Océanologique de Banyuls
de Banyuls, INRS. BP 44, F-66651 Banyuls-sur-mer, cedex, France. Phone:
Phone: 33-4-68-88-73-53, Fax: 33-4-68-88-73-95, email: email@example.com
Océanologique de Banyuls-sur-Mer (OOB) is one of the more important
research centers along the French Mediterranean coast for biological marine
sciences. Approximately 110 persons are currently appointed at the OOB.
OOB has a large variety of instrumentation and is operating modernly equipped
research vessels for coastal and ocean studies. The institute is an European
Associated Laboratory (LEA) linking our laboratory and the University of
Barcelona as well as the Spanish national research organization (CSIC).
The OOB is part of the centre national de la Recherche Scientifique (CNRS)
and within the Institut National des Scienes de líUnivers (INSU): it is
located on the NW Mediterranean coast, close to Spain and at 200 kms of
Barcelona. This area is an oligotrophic area in contrast to the Barcelona
coast which is submitted to an important anthropogenic pressure. Therefore,
both sites are very different and travels between the two sampling sites
can be fast and within 2 hours by car.
Within OOB, the research unity of Biological
Oceanography has a large range of analytical facilities for microbial (including
bacteria and picophytoplankton) and molecular ecology studies including
microcosms. Members from this unity have been involved in previous research
projects on eutrophication and productivity of coastal ecosystems funded
by different agencies including the EU. All the equipment necessary for
successfully executing this project is available
Selected references relevant to
2001 Bernard L., Courties C., Muyzer G. and Lebaron P. A new approach combining
cellular and molecular techniques with cell sorting to relate genetic and
physiological diversity within natural communities. Cytometry 43 : 314-321.
2001 Lebaron, P., Agogué, H., Courties, C., Servais, P. and Joux,
F. Does the high nucleic acid content of individual bacterial cells allow
us to discriminate between active cells and inactive cells in aquatic systems.
Appl. Environ. Microbiol. 67 :1775-1782.
2001 Schäfer H., Bernard L., Courties C., Lebaron P., Servais P.,
Pukall R., Stackebrandt E., Troussellier M., Guindulain T., Vives-Rego
T., Muyzer G. Microbial community dynamics in Mediterranean nutrient
enriched seawater mesocosms : changes in the genetic diversity and identity
of bacterial populations. FEMS Microb. Ecol., 34 : 243-253.
2001 Servais, P., Agoghé, H., Courties, C., Joux, F. and Lebaron
P. Are the actively respiring cells (CTC+) those responsible of bacterial
production in aquatic environments ? FEMS Microb. Ecol. 35 :171-179.
2000 Bernard L., Courties C., Servais P., Trousellier M., Petit M. and
Lebaron P. Relationships among bacterial cell size, productivity and genetic
diversity in aquatic environments using cell sorting and flow cytometry.
Microb. Ecol. 40:148-158.
2000 Bernard L., Schäfer H., Joux F., Courties C., Muyzer G., and
Lebaron P. Genetic diversity of total, active and culturable marine bacteria
in coastal waters. Aquat. Microb. Ecol. 23 :1-11.
2000 Ghiglione J-F., Gourbière, F., Potier P. Philippot, P., Lensi,
R. Role of the respiratory nitrate reductase in ability of Pseudomonads
fluorescens YT101 to colonize the rhizosphere of maize. Appl. Environ.
Microbiol. 66 :4012-4016.
2000 Joux F. and Lebaron P. Use of fluorescent probes to assess physiological
functions of bacteria at the single cell level. Microbes and Infection.
2000 Vives-Rego J., Lebaron P., and Nebe-Von Caron G. Current and future
applications of flow cytometry in aquatic microbiology. FEMS Microb. Rev.
1999 Joux F., Jeffrey W.H., Lebaron P. and D. Mitchell. Diversity of responses
among marine bacteria during UV-B exposure and subsequent recoveries. Appl.
Environ. Microbiol. 65 : 3820 ? 3827.
1999 Lebaron P., Servais P., Troussellier M., Courties C., Vives-Rego J.,
Muyzer G., Bernard L., Guindulain T., Schäfer H., Pukall R., Stackebrandt
E. Changes in bacterial community structure in seawater mesocosms differing
in their nutrient status. Aquatic Microbial. Ecol. 19 : 255 ? 267.
1999 Pukall R., Pâuker O., Buntufeu D., Ulrichs G. Lebaron P., Bernard
L., Guindulain T., Vives-Rego J. and Stackebrandt E. High sequence diversity
of Alteromonas macleodii-related cloned and cellular 16S rDNA from a Mediterranean
seawater mesocosm experiment. FEMS Microb. Ecol. 28 : 335-344.
1999 Servais P., Courties C., Lebaron P., Trousellier M. Coupling bacterial
activity measurements with cell sorting by flow cytometry. Microb. Ecol.,
38 : 180-189.
1999 Troussellier M., Courties C., Lebaron P., Servais P. Flow cytometric
discrimination of bacterial subpopulations in seawater based on SYTO 13
staining of nucleic acids. FEMS Microbiol. Ecol. 29 :319-330.
1998 Lebaron P., Parthuisot N., and Catala P. Comparison of blue nucleic
acid dyes for enumeration of total bacteria in aquatic systems. Appl. Environ.
Microbiol. 64 : 1725-1730