PARTNER#2 Observatoire Océanologique de Banyuls  OOB France

Observatoire Océanologique de Banyuls, INRS. BP 44, F-66651 Banyuls-sur-mer, cedex, France. Phone: Phone: 33-4-68-88-73-53, Fax: 33-4-68-88-73-95, email:
Institution profile The Observatoire Océanologique de Banyuls-sur-Mer (OOB) is one of the more important research centers along the French Mediterranean coast for biological marine sciences. Approximately 110 persons are currently appointed at the OOB. OOB has a large variety of instrumentation and is operating modernly equipped research vessels for coastal and ocean studies. The institute is an European Associated Laboratory (LEA) linking our laboratory and the University of Barcelona as well as the Spanish national research organization (CSIC). The OOB is part of the centre national de la Recherche Scientifique (CNRS) and within the Institut National des Scienes de líUnivers (INSU): it is located on the NW Mediterranean coast, close to Spain and at 200 kms of Barcelona. This area is an oligotrophic area in contrast to the Barcelona coast which is submitted to an important anthropogenic pressure. Therefore, both sites are very different and travels between the two sampling sites can be fast and within 2 hours by car. 

Within OOB, the research unity of Biological Oceanography has a large range of analytical facilities for microbial (including bacteria and picophytoplankton) and molecular ecology studies including microcosms. Members from this unity have been involved in previous research projects on eutrophication and productivity of coastal ecosystems funded by different agencies including the EU. All the equipment necessary for successfully executing this project is available

Prof. Philippe Lebaron Chief Scientist Univ. Group coordination
Dr. Jean-François Ghiglione Senior Scientist CNRS Molecular biology and diversity assessment
Nicole Bourrain Researcher at Pierre Fabre Dermocosmetique Microbial ecology
Dr. Fabien Joux Senior scientist-Univ PMC, Paris VI Microbial ecology and UV radiations
Marielle Larcher Postdoctoral scientist  
Philippe Catala Engineer CNRS Cytometry and cell sorting
Laurent Intertaglia Technitian
Nicole Batallier Technitian- Univ. PMC-Paris VI


Selected references relevant to BASICS

  • 2001 Bernard L., Courties C., Muyzer G. and Lebaron P. A new approach combining cellular and molecular techniques with cell sorting to relate genetic and physiological diversity within natural communities. Cytometry 43 : 314-321.
  • 2001 Lebaron, P., Agogué, H., Courties, C., Servais, P. and Joux, F. Does the high nucleic acid content of individual bacterial cells allow us to discriminate between active cells and inactive cells in aquatic systems. Appl. Environ. Microbiol. 67 :1775-1782.
  • 2001 Schäfer H., Bernard L., Courties C., Lebaron P., Servais P., Pukall R., Stackebrandt E., Troussellier M., Guindulain T., Vives-Rego T., Muyzer G. Microbial community dynamics in Mediterranean nutrient  enriched seawater mesocosms : changes in the genetic diversity and identity of bacterial populations. FEMS Microb. Ecol., 34 : 243-253.
  • 2001 Servais, P., Agoghé, H., Courties, C., Joux, F. and Lebaron P. Are the actively respiring cells (CTC+) those responsible of bacterial production in aquatic environments ? FEMS Microb. Ecol. 35 :171-179.
  • 2000 Bernard L., Courties C., Servais P., Trousellier M., Petit M. and Lebaron P. Relationships among bacterial cell size, productivity and genetic diversity in aquatic environments using cell sorting and flow cytometry. Microb. Ecol. 40:148-158.
  • 2000 Bernard L., Schäfer H., Joux F., Courties C., Muyzer G., and Lebaron P. Genetic diversity of total, active and culturable marine bacteria in coastal waters. Aquat. Microb. Ecol. 23 :1-11.
  • 2000 Ghiglione J-F., Gourbière, F., Potier P. Philippot, P., Lensi, R. Role of the respiratory nitrate reductase in ability of Pseudomonads fluorescens YT101 to colonize the rhizosphere of maize. Appl. Environ. Microbiol. 66 :4012-4016.
  • 2000 Joux F. and Lebaron P. Use of fluorescent probes to assess physiological functions of bacteria at the single cell level. Microbes and Infection. 2 :1523-1535.
  • 2000 Vives-Rego J., Lebaron P., and Nebe-Von Caron G. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microb. Rev. 24 :429-448.
  • 1999 Joux F., Jeffrey W.H., Lebaron P. and D. Mitchell. Diversity of responses among marine bacteria during UV-B exposure and subsequent recoveries. Appl. Environ. Microbiol. 65 : 3820 ? 3827.
  • 1999 Lebaron P., Servais P., Troussellier M., Courties C., Vives-Rego J., Muyzer G., Bernard L., Guindulain T., Schäfer H., Pukall R., Stackebrandt E. Changes in bacterial community structure in seawater mesocosms differing in their nutrient status. Aquatic Microbial. Ecol. 19 : 255 ? 267.
  • 1999 Pukall R., Pâuker O., Buntufeu D., Ulrichs G. Lebaron P., Bernard L., Guindulain T., Vives-Rego J. and Stackebrandt E. High sequence diversity of Alteromonas macleodii-related cloned and cellular 16S rDNA from a Mediterranean seawater mesocosm experiment. FEMS Microb. Ecol. 28 : 335-344.
  • 1999 Servais P., Courties C., Lebaron P., Trousellier M. Coupling bacterial activity measurements with cell sorting by flow cytometry. Microb. Ecol., 38 : 180-189.
  • 1999 Troussellier M., Courties C., Lebaron P., Servais P. Flow cytometric discrimination of bacterial subpopulations in seawater based on SYTO 13 staining of nucleic acids. FEMS Microbiol. Ecol. 29 :319-330.
  • 1998 Lebaron P., Parthuisot N., and Catala P. Comparison of blue nucleic acid dyes for enumeration of total bacteria in aquatic systems. Appl. Environ. Microbiol. 64 : 1725-1730